fluorescent cell meaning in Chinese
荧光试池,荧光细胞
Examples
- Fluorescent cells were not detected in tonsil, liner, larynx, and kidney .
在扁桃体、肝、喉部和肾脏中没有查到荧光细胞。 - The plasmid ploxgfp dna was transfected into bovine fetal fibroblast cells , then on the selected g418 medium , the green fluorescent cell clones were gained
将鸡-干扰素cdna克隆到载体plox上的loxp和lox511序列之间,构建成另一打靶载体? ploxifn 。 - After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut
经pcr检测后,有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带,表明发生了替换反应;另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用,剪切了gfp基因,从而只扩增出了载体序列和一个lox位点的约500bp的条带。 - After multiple , plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones , then filtrated the non - fluorescent cell clones . two non - fluorescent cell clones , the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr
首先将质粒ploxgfpdna电转染牛胎儿成纤维细胞,并用g418筛选,筛选出绿色荧光细胞克隆,增殖培养之后,将质粒ploxifn和pbs185dna共转染荧光细胞克隆,筛选出不发光的克隆。 - Using the green fluorescent cell clones as the template , a product of 4kb which was the same as the result of pcr of the plasmid ploxgfp was got . on the other hand , not only a 4kb product but also a 1000bp product and a 500bp product were showed when the co - transfected cells were used to be the template
以绿色荧光细胞克隆为模板扩增出了包括gfp基因、 neo基因等在内的约4kb的条带;以共转染后的未经筛选的细胞为模板既扩增出了约1000bp的条带,又扩增出了约4kb和500bp的条带。